Journal: Discover. Oncology

Article Title: Cyclovirobuxine D inhibits hepatocellular carcinoma growth by inducing ferroptosis of hepatocellular carcinoma cells

doi: 10.1007/s12672-024-00940-2

Figure Lengend Snippet: CVB-D induced Ferroptosis in HepG2 and Huh-7 cells. HepG2 and Huh-7 cells were exposed to different doses of CVB-D (60, 80 and 100 µM) for 48 h. A The level of ROS was detected by fluorescent probe DCFH-DA. B The level of MDA was detected by lipid peroxidation MDA assay kit. C The protein expression of GPX4 and FSP1 was detected by western blotting. The amount of protein in term of the band intensity was analyzed by ImageJ. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. control group

Article Snippet: The primary antibodies were diluted into 5% nonfat milk as follows: Bax antibody (ab32503, Abcam, UK, 1:5000), Bcl-2 antibody (WL01556, Wanleibio, China, 1:500), GPX4 antibody (ab125066, Abcam, UK, 1:1000), FSP1 antibody (342551, Zenbio, China, 1:1000), and GAPDH antibody (ab137959, Absin, China, 1:5000).

Techniques: Multiple Displacement Amplification, Expressing, Western Blot, Control

Journal: Discover. Oncology

Article Title: Cyclovirobuxine D inhibits hepatocellular carcinoma growth by inducing ferroptosis of hepatocellular carcinoma cells

doi: 10.1007/s12672-024-00940-2

Figure Lengend Snippet: Inhibition of ferroptosis by Fer-1 attenuates cell death induced by CVB-D in HCC cells. HepG2 and Huh-7 cells were treated with or without 100 µM CVB-D and 2 µM Fer-1 for 48 h. A The cell viability was measured by MTT assay. B The ferrous ion content was detected by a ferrous ion content assay kit. C The level of ROS was detected by fluorescent probe DCFH-DA. D The level of MDA was detected by lipid peroxidation MDA assay kit. E The protein expression of GPX4 and FSP1 was detected by western blotting. The amount of protein in term of the band intensity was analyzed by ImageJ. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. CVB-D group

Article Snippet: The primary antibodies were diluted into 5% nonfat milk as follows: Bax antibody (ab32503, Abcam, UK, 1:5000), Bcl-2 antibody (WL01556, Wanleibio, China, 1:500), GPX4 antibody (ab125066, Abcam, UK, 1:1000), FSP1 antibody (342551, Zenbio, China, 1:1000), and GAPDH antibody (ab137959, Absin, China, 1:5000).

Techniques: Inhibition, MTT Assay, Multiple Displacement Amplification, Expressing, Western Blot